HOME | Research | Media | Careers | Contacts | Products | Search | Publications | Site Map
CSIRO Mathematics, Informatics and Statistics

 

 

Image Analysis
Biotech Imaging Group
Application Areas
 Biotechnology
 Cellular Screening
 Health
 Asset Monitoring
 Exploration
 Other Areas
Skills
 Segmentation
 Feature Extraction
 Statistical Analysis
 Stereo Vision
 Image Motion
 
Projects
Imaging Services
Imaging Products
Track Record
Publications
Patents
Staff

Define the basic geometry of a batch of microarray images

Description

`SetParameters' is an interactive function for specifying the basic geometric structure of a set of microarray images. The array dimensions can be specified by the arrangement of grids (e.g. 4x4) and the arrangements of spots within grids (e.g. 19 x21).

Usage

SetParameters(batch)

Details

The user is prompted for values for the following parameters:

nspot.r
the number of rows of spots per grid;
nspot.c
the number of columns of spots per grid;
ngrid.r
the number of rows of grids per image;
ngrid.c
the number of columns of grids per image;
tolerance.r
top / bottom translation tolerance;
tolerance.c
left / right translation tolerance.

The last two of these parameters correspond to the amount of positional variation expected between images in the batch. See the Spot user's guide http://www.cmis.csiro.au/iap/Spot/spotmanual.htm

Value

None.
A file named "parameters.<batch>" containing the specified information is created in the current directory.

Author(s)

Jean Yang

References

Spot user's guide. http://www.cmis.csiro.au/iap/Spot/spotmanual.htm

See Also

SetTemplate, Spots, SpotRGB

Examples

> SetParameters("array1")
Enter number of rows of grids per image (ngrid.r): 4
Enter number of columns of grids per image (ngrid.c): 4
Enter number of rows of spots per grid (nspot.r): 19
Enter number of columns of spots per grid (nspot.c): 21
Enter top/bottom translation tolerance, default is 50 (tolerance.r): 20
Enter left/right translation, default is 50 (tolerance.c): 30
Initialization complete

© Copyright 2012, CSIRO Australia
Use of this web site and information available from
it is subject to our
Legal Notice and Disclaimer and Privacy Statement