Translocation Assays
We have developed automated solutions for the translocation assays
commonly used in target identication/validation and compound screening
in drug discovery.
We have research collaborations in which we have custom developed
solutions for Nucleus-Cytoplasm translocation
for more
difficult primary cells.
Commercial Partners
Our conventional translocation analysis code has been integrated into
the HCA cellular imaging systems of:
- Molecular Devices (formerly Axon Instruments) (http://www.axon.com)
are a major biotechnology instrumentation company that CSIRO has worked
with in the development of their
ImageXpressTM
cellular screening system.
If you would like to licence this module for integration into your
software platform please see the contact details below.
Custom Solutions
We develop custom solutions for non-standard assays. If
you'd like a quote please see the contact details below.
Nucleus-Cytoplasm translocation for commonly used cell
lines
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A typical cytoplasm channel image with densely
packed cells from a common cell line. When well separated, these
cells are roughly elliptical in shape and the cytoplasm is
symmetrically located around the nucleus. |
In widefield microscopy, it is difficult to
segment the cytoplasm directly. So cytoplasm measurements are
usually taken using a surrogate for the cytoplasm - an isotropic
"donut" region around the nucleus. |
When cells are densely packed, isotropic donuts
will include some background. Our solution allows the use of
anisotropic "donuts". This reduces the likelihood that low
signal background will bias the cytoplasm measurement. |
Nucleus-Cytoplasm
translocation for more difficult primary cells
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A typical cytoplasm channel image with densely
packed primary cells. Even when well separated, these cells are not
elliptical in shape and nor is the cytoplasm symmetrically located
around the nucleus. |
We have developed a new cytoplasm surrogate using
adaptive donuts. These expand within the cytoplasm differentially to
avoid the background. This significantly reduces the false alarm
rate from incorrect cytoplasm measurements. |
Cytoplasm-vesicle
translocation
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typical cytoplasm-to-vesicle translocation assay – a negative
result is shown above, a positive result is shown below. |
Standard
solutions just count the vesicles/dots in the full image. So
heterogeneous responses cannot be detected. |
Our
solution allocates each vesicle/dot to a single cell. So atypical
cells with high or low response can be detected. |
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For further information, please contact:
Pascal Vallotton
Leader, Biotech Imaging
CSIRO Mathematics, Informatics and Statistics
Locked Bag 17, North Ryde NSW 1670 AUSTRALIA
Phone: +61 (0)2 9325 3208
Fax: +61 (0)2 9325 3200
Email: pascal.vallotton@csiro.au
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Leanne Bischof
Biotech Imaging
CSIRO Mathematics, Informatics and Statistics
Locked Bag 17, North Ryde NSW 1670 AUSTRALIA
Phone: +61 (0)2 9325 3206
Fax: +61 (0)2 9325 3200
Email: leanne.bischof@csiro.au |
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